Staff frantically battled to save them after a woman raised the alarm when she stepped on one of the fish which had jumped out of its tank.
Workers then found one of their most expensive tanks had a strong chemical smell and the area was evacuated.
The discus and plecos fish at World of Water, in Studley Grange Garden Centre near Swindon, Wilts, could not be saved.
A spokesman described it as "pointless cruelty", confirming that they will upgrade security and police had been contacted.
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