Murray cast doubt over his participation at the All England Club in just 11 days after pulling out of his second-round match at Queen's Club against Jordan Thompson.
The 37-year-old had not looked right from the outset and stayed rooted to the spot after one early serve, unable to put any weight on his right leg.
He underwent a medical timeout face down on court at 2-1 down and carried on for two further games before reluctantly withdrawing at what is his final appearance at Queen's, a tournament he has won a record five times.
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