The claims, made in US court papers, relate to thousands of transactions between 2008 and 2013 and allege that sanctions against Iran were broken.
They include foreign exchange deals worth about $9.6 billion involving people or companies that the US government linked with funding Hamas, Hezbollah, the Taliban and al Qaeda.
The whistleblowers claim that the transactions at the centre of the latest allegations were found after they re-examined material previously sent to US authorities in 2012.
They are seeking to revive the case, which ended in Standard Chartered paying $227 million for breaking US law by moving millions of dollars for groups in Iran and other countries when under sanctions. The bank's settlement allowed it to avoid criminal charges.
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