The industrial action follows a similar two-day strike last week, which was the first at the Guardian in more than 50 years.
National Union of Journalists (NUJ) members passed a motion in November stating that selling the Sunday newspaper to Tortoise would be a "betrayal" of the Scott Trust's commitment to the Observer.
The trust is the ultimate owner of Guardian Media Group. Last Friday it was announced that a deal to sell the Observer, the world's oldest Sunday newspaper, to Tortoise Media, had been agreed in principle.
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