Assisted Reproductive Technology (ART) has made immense strides since the first live birth following In-vitro Fertilisation (IVF) in 1978. This multi-faceted improvement led to the optimisation of ovarian stimulation and a better assessment of embryo quality, giving rise to steady increase in live birth delivery rates.
The cumulative live birth rate after one oocyte retrieval cycle is considered the most accurate benchmark for ART success.
Empirical evidence shows that embryo evaluation based on morphology, morphokinetics, preimplantation genetic testing (PGT-A) or embryo metabolism, and microfluidics, all have the potential to rank embryos according to implantation potential.
We do not have high quality data showing the difference in ranking potential between these different methods to evaluate embryo quality. However, much of ongoing studies are based on these. The aim is to improve live birth rate or cumulative birth rate for most recent developments in ART.
IVF lab A perfect homeostasis is provided by in vivo micro environment during the course of blastocyst development. The same is mimicked in IVF lab.
Various commercial media companies recommend differing pH ranges, most within the range of 7.2–7.4. Most don’t reveal the secret composition of media and fall short of robust research to indicate best media composition and incubation parameters.
Historically, CO2 set-points in incubators are adjusted to attempt to create the ideal media pH environment for any development stage of embryos. CO2 adjustments are usually done by calibrating the pH of equilibrated media once a month or once a week. There are many gadgets for pH measurement for IVF culture systems. A bench top pH meter can be used for the routine pH measurement with proper calibration buffers.
However, it is essential to keep the certificate of analysis of the media being used. A new technology, SAFE Sens Trak Station, enables real time non-invasive pH monitoring within an incubator environment.
Use of this technology can reveal about incubator conditions which are not ideal for maintaining ideal pH levels. Use of RFID tags precludes the errors in the lab.
Bu hikaye eHEALTH dergisinin December 2018 sayısından alınmıştır.
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Bu hikaye eHEALTH dergisinin December 2018 sayısından alınmıştır.
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